The aerial parts of Cassia auriculata were weighed accurately and defatted With petroleum ether. The defatted mare was then refluxed with 70% ethanol for about 5-6 hrs. The 70% ethanolic (Flavonoid rich fraction) extract of the plant of Cassia auriculata was then subjected for the evaluation of antimicrobial activity and for the estimation of Total Flavonoid Contents. 70° 0 ethanolic (flavonoid rich fraction) extract of the plant of Cassia auriculata were subjected for the determination of antifungal and antibacterial activities.